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RD Recombinant human IL-17A人白介17因子

  • 更新時(shí)間:  2023-07-24
  • 產(chǎn)品型號:  314-ml-025
  • 簡(jiǎn)單描述
  • RD Recombinant human IL-17A人白介17因子
    Source E. coliderived
    此產(chǎn)品還有小鼠IL-17A
詳細介紹

RD Recombinant human IL-17A人白介17因子

DESCRIPTION
Source E. coliderived
Thr22Ala158
Accession # Q62386.1
Nterminal
Sequence
Analysis
Thr22
Structure / Form Disulfidelinked
homodimer
Predicted Molecular
Mass
15.5 kDa (monomer)
SPECIFICATIONS
Activity Measured by its ability to induce IL6
secretion by NIH-3T3 mouse embryonic fibroblast cells. Yao, Z. et al. (1995) Immunity 3:811.
The ED50 for this effect is typically 0.251.25
ng/mL.
Endotoxin Level <1.0 EU per 1 μg of the protein by the LAL method.
Purity >97%, by SDSPAGE
under reducing conditions and visualized by silver stain.
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. See Certificate of Analysis for details.
PREPARATION AND STORAGE
Reconstitution Reconstitute at 25 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediay at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freezethaw
cycles.
l 12 months from date of receipt, 20
to 70
°C as supplied.
l 1 month, 2 to 8 °C under sterile conditions after reconstitution.
l 3 months, 20
to 70
°C under sterile conditions after reconstitution.
BACKGROUND
Interleukin 17 (also known as CTLA8)
is a T cellexpressed
pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of
herpesvirus Saimiri. cDNA clones encoding IL17
have been isolated from activated rat, mouse and human T cells. Mouse IL17
cDNA encodes a 158 amino acid (aa)
residue precursor protein with a 21 amino acid residue signal peptide that is cleaved to yield the 137 aa residue mature IL17.
Both recombinant and natural IL17
have
been shown to exist as disulfide linked homodimers. At the amino acid level, mIL17
shows 57% and 87% sequence identity with herpesvirus and rat IL17,
respectively. An IL17
specific mouse cell surface receptor (IL17
R) has been cloned. While the expression of IL17
mRNA is restricted to activated alpha beta
TCR+CD4CD8T
cells, the expression of mIL17
R mRNA has been detected in virtually all cells and tissues tested. IL17
exhibits multiple biological activities on a
variety of cells including: the induction of IL6
and IL8
production in fibroblasts? the enhancement of surface expression of ICAM1
in fibroblasts? activation of NFκB
and costimulation of T cell proliferation.
References:
1. Kennedy, J. et al., (1996) J. Interferon Cytokine Res. 16:611.
2. Yao, Z. et al., (1995) J. Immunol. 155:5483.
3. Yao, Z. et al., (1995) Immunity 3:811.
4. Rouvier, E. et al., (1993) J. Immunol. 150:5445.

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