九月婷婷人人澡人人添人人爽,小雪被老汉玩各种方式,久久精品国产亚洲AV成人,老妇女性较大毛片

您好,歡迎進(jìn)入上海力敏實(shí)業(yè)有限公司網(wǎng)站!
一鍵分享網(wǎng)站到:
產(chǎn)品搜索
PRODUCT SEARCH
產(chǎn)品分類(lèi)
PRODUCT CLASSIFICATION
相關(guān)文章
RELEVANT ARTICLES
您現在的位置:首頁(yè) >> 產(chǎn)品中心 >> 生化試劑、抗體、血清 >> 常規生化試劑 >> sigma現貨代理Dispase II D-4693

sigma現貨代理Dispase II D-4693

  • 更新時(shí)間:  2023-07-26
  • 產(chǎn)品型號:  
  • 簡(jiǎn)單描述
  • sigma現貨代理Dispase II D-4693
    Related CategoriesApplication Index, Biochemicals and Reagents, Cell Biology, Dissociation, Enzymes, Inhibitors, and Substrates,
    More...
    activity ≥0.5 units/mg solid
    sol
詳細介紹

sigma現貨代理Dispase II D-4693

Description

Analysis Note

The enzyme can be initially dissolved in 50 mM Hepes/KOH pH 7.4, 150 mM NaCl at 10 mg/ml. Further dilutions should be made in the cell culture media most appropriate for the cells being dissociated. Dispase is a metalloproteinase and is activated by divalent cations including Ca2+ , Mg2+, Mn2+ and Fe2+.

Preparation Note

A 50 U/mL stock solution may be prepared by dissolving the powder in a buffer containing 10 mM NaAc (pH 7.5) and 5 mM CaAc. It should be stored at 4 °C.

Unit Definition

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent), unless otherwise indicated.

Physical form

lyophilized powder containing calcium acetate and milk sugar

Application

The enzyme from Sigma has been used in developing a protocol for ex vivoculture of mouse embryonic mammary buds.[5] It has been used in the treatment of rat heart pieces during the isolation of mitochondria from rat heart.[6] It has also been used for the isolation of dental pulp stem cells (DPSCs) by enzymatic hydrolysis. These cells have been compared with DPSCs isolated by explant method to analyse their stem cell and differentiation properties.[1]
Dispase II has been used for Fluorescence-Activated Cell Sorting (FACS).[3] It has also been used for separating visceral yolk sac layers.[2]

Biochem/physiol Actions

Dispase II is a neutral protease that hydrolyzes the N-terminal peptide bonds of non-polar amino acid residues. It may be used for separating many tissues and cells grown in vitro. The enzyme is very gentle and does not damage cell membranes. It can also be used to prevent clumping in suspension cultures. This protease cleaves fibronectin and type IV collagen, but not laminin, type V collagen, serum albumin, or transferrin.[4] Dispase II is specific for the cleavage of Leucine-Phenylalanine bonds. Ca2+, Mg2+, Mn2+, Fe2+, Fe3+ and Al3+activate the enzyme. EDTA, EGTA, Hg2+ and other heavy metals inhibit the enzyme activity.[7] The enzyme contains 1g-atom of zinc per g-mol of purified enzyme. If this zinc component is removed by chelating agents such as EDTA or EGTA, an inactive apoenzyme is obtained. The enzyme is not inhibited by serum.

sigma現貨代理Dispase II D-4693

我公司代理sigma公司試劑,大量現貨,和選購


留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話(huà):

  • 常用郵箱:

  • 省份:

  • 詳細地址:

  • 補充說(shuō)明:

  • 驗證碼:

    請輸入計算結果(填寫(xiě)阿拉伯數字),如:三加四=7
桦川县| 天全县| 都兰县| 洪洞县| 广丰县| 扬中市| 青海省| 怀集县| 通道| 富阳市| 吉安县| 桐梓县| 迁西县| 潍坊市| 水城县| 台南市| 四子王旗| 丰城市| 夏津县| 兰考县| 黎川县| 浦城县| 上犹县| 黎城县| 桐梓县| 普宁市| 玉山县| 桓仁| 九寨沟县| 汝阳县| 伊宁市| 漳州市| 曲水县| 灯塔市| 曲阜市| 凯里市| 宣武区| 琼中| 巧家县| 会宁县| 突泉县|